Snow lotus polyploid inducing process

雪莲多倍体的诱导方法

Abstract

本发明涉及一种雪莲多倍体的诱导方法,该方法是针对雪莲特殊的生理特性,通过雪莲种子萌发产生无菌苗,然后将切除根叶后保留下来的无菌苗茎干部分接种于添加有二甲基亚砜和秋水仙素的出芽诱导培养基上进行诱导后,再转移到不含二甲基亚砜和秋水仙素的出芽诱导培养基上进行诱导出芽,经过秋水仙素处理的无菌苗抽出新芽,植株外观与对照苗差异显著,然后对无菌苗的茎尖生长点做染色体检测以确定染色体的倍性,从而得到了雪莲多倍体植株。
The snow lotus polyploid inducing process includes the first germinating snow lotus seed to generate aseptic seedling, the subsequent cutting off the roots and leaves of the aseptic seedling, inoculating the stem of the aseptic seedling first to culture medium with dimethyl sulfoxide and colchicina and then to culture medium without dimethyl sulfoxide and colchicina to induce germination so as to obtain new sprout and new distinctly different plant, and finally chromosome detection on the stem apex growing point of the aseptic seedling to determine ploidy of the chromosome and obtain snow lotus polyploid plant.

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Cited By (3)

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    CN-102893860-AJanuary 30, 2013崔广荣一种甜叶菊多倍体离体诱导技术
    CN-103518622-AJanuary 22, 2014西北大学一种低大气压力延长新疆雪莲试管苗在低海拔生存时间的方法
    CN-103518622-BJanuary 20, 2016西北大学一种低大气压力延长新疆雪莲试管苗在低海拔生存时间的方法