具有ω3脂肪酸去饱和活性的多肽及编码其多肽的多核苷酸及其利用

Polypeptide having activity of unsaturating omega3-fatty acid, polynucleotide coding for the polypeptide and use thereof

Abstract

本发明提供具有广泛的底物特异性,具有在ω3位高效生成不饱和键的ω3脂肪酸去饱和活性的多肽及编码其多肽的多核苷酸。通过使此多核苷酸在生物体内表达,可大量生产n-3系列PUFAs。具有ω3脂肪酸去饱和活性的多肽且是由序列号:1所示的氨基酸序列组成的多肽,编码具有ω3脂肪酸去饱和活性的多肽的多核苷酸且是由序列号:2或3所示的碱基序列组成的多核苷酸等,均对n-3系列脂肪酸的生产起作用。
A polypeptide that exhibits wide substrate specificity, having the activity of unsaturating 3-fatty acids capable of efficiently forming an unsaturated bond at the 3-position; and a polynucleotide coding for the polypeptide. Mass production of n-3 PUFAs can be realized by in vivo expression of this polynucleotide. For example, a polypeptide having the activity of unsaturating 3-fatty acids and consisting of an amino acid sequence of SEQ ID NO: 1 and a polynucleotide coding for the polypeptide having the activity of unsaturating 3-fatty acids and consisting of a base sequence of SEQ ID NO: 2 or 3 are useful for the production of n-3 fatty acids.

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